科研之友
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摘要
The effects of pretreatment methods of piggery sewage, types and light path of piping photoreactors on the growth of Chlorella pyrenoidosa and purification of piggery sewage were systematically studied under outdoor conditions. The results showed that when the COD, NH_4^+, PO_4^(3-), TN and TP of raw swine wastewater were 710, 491, 54, 590 and 108 mg?L-1, respectively, Chlorella pyrenoidosa performed the best purification of the tertiary filtration pretreated wastewater, and the removal rates of colority and NH_4^+ reached 88.56% and 83.48%, respectively, which were significantly higher than those for the raw wastewater treatment (P<0.05). Under outdoor conditions in summer, the scale up culture of Chlorella pyrenoidosa with plastic pipes indicated that these algae could withstand high temperature and grow well with a yield (dry weight) of 0.13 g?L-1. When Chlorella pyrenoidosa was cultured in a vertical photobioreactor with a diameter of 5 cm, the yield (dry weight) was 0.93 g?L-1 and the highest protein content was 58.9% through recycling collection. The contents of mercury, arsenic, cadmium and lead in algal biomass were lower than 0.1, 1.0, 0.5 and 4.0 mg?kg-1, respectively, which was in accordance with the standard Chlorella Powder for Feed (DB32/T 564-2010). The removal rates of NH3-N, PO_4^(3-), and colority in effluent after harvesting Chlorella were all over 90%, which basically met the national emission standards. The results of this study can provide references for technology development of piggery sewage purification and industrial production of Chlorella pyrenoidosa.
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