The effect of hyperuricemia on cardiac function, nuclear factor‐κB in myocardial tissue, Mn superoxide dismutases activity in mitochondria of rats

作者：Chunyan Hao; Hubin Duan; Junfeng Zhang; Suping Wang; Xuejun Liu; Shengyan Cao; Xiaojun Zheng; Ling Gao; Lijuan Pan; cuiming Liu

来源：The FASEB Journal, 2018, 32.

DOI：10.1096/fasebj.2018.32.1_supplement.674.1

• Hyperuricemia
• NF‐kappa B
• Superoxide dismutase
• Heart function tests
• Mitochondria

Objective(#br)To investigate the effect of hyperuricemia (HUA)on nuclear factor‐κB(NF‐κB) in myocardial tissue, Mn superoxide dismutases ( Mn‐SOD) activity in mitochondria, cardiac function of rats with high blood uric acid (HUA).(#br)Methods(#br)Sixty SD male rats, including 30 young rats and 30 aged rats, were randomly divided into four groups: the young control group, the young HUA group, the aged control group and the aged HUA group. Fifteen rats were in each group. The HUA rat models were set up by lavage method with yeast extract and ethambutol. Left ventricular systolic pressure (LVSP), left ventricular end‐diastolic pressure (LVEDP) and left ventricular pressure at the end of the rising and falling maximum velocity (+ dp/dtmax) were determinated by intubation in the left ventricular with cardiac catheter. Mn‐SOD activity in the mitochondria of rats myocardial tissue were detected by chemical colorimetry; The expression of nuclear NF‐κB in rats myocardial tissue were measured by immunohistoc...