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摘要
Directed evolution provides a simple and high-efficiency tool for the development of synthetic biology, especially in the chemical synthesis and medicine. However, the traditional directed evolution technique has the problems of cumbersome operation, time-consuming and low-efficiency, which cannot satisfy the construction and screening of mass mutant libraries. In recent years, a technique about in vivo continuous directed evolution that seamlessly integrates mutation, translation (if the evolving molecules are not genes themselves), screening and replication processes into an uninterrupted cycle has emerged, which makes breakthrough in phage, bacteria and eukaryotic cells, greatly facilitating technological innovation of directed evolution. Simultaneously, with the development of in vivo continuous evolution technology, screening methods and evolutionary devices are also constantly improved. Here, this review is done to expound the latest research progress on continuous directed evolution techniques, screening methods and devices, and discuss the current challenges and opportunities. ? 2021 China Biotechnology Press.
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