IGF-1 regulates cAMP levels in astrocytes through a 汕2-adrenergic receptor-dependant mechanism

作者：Daniel Chesik; Nadine Wilczak; Jacques De Keyser

来源：International Journal of Medical Sciences, 2008.

We have recently demonstrated that neonatal astrocytes derived from mice lacking beta-2 adrenergic receptors (汕2AR) possess higher proliferation rates, as compared to wild-type cells, an attribute that was shown to involve insulin-like growth factor (IGF) signaling. In the present study, we demonstrate that basal cAMP levels in 汕2AR knockout astrocytes were significantly lower than in wild type cells. Furthermore, treatment with IGF-1 reduced intracellular cAMP levels in wild type astrocytes, yet had no effects on cAMP levels in 汕2AR deficient astrocytes. Our data suggests that IGF-1 treatment influences cAMP production through a 汕2AR-dependant mechanism in astrocytes. A deficit of 汕2AR on astrocytes, as previously reported in multiple sclerosis, may influence cell proliferation, an action which could have implications in processes involved in astrogliosis.