Synthesis of Autophagosomal Marker Protein LC3-II under Detergent-Free Conditions

作者:Huang, Yi-Chao; Li, Yi-Ming; Chen, Yang; Pan, Man; Li, Yi-Tong; Yu, Li; Guo, Qing-Xiang; Liu, Lei*
来源:Angewandte Chemie - International Edition, 2013, 52(18): 4858-4862.
DOI:10.1002/anie.201209523

摘要

Just add oil: A new detergent-free method was developed to synthesize lipidated proteins using a light-activatable solubilizing side chain (in dashed circle, see scheme) to assist the ligation of the lipopeptides. This method allows the efficient preparation of a phosphatidylethanolamine-conjugated autophagosomal marker protein (LC3-II) as well as labeled derivatives of LC3-II, which can be used to study autophagy regulation.