汕-Sitosterol: Supercritical Carbon Dioxide Extraction from Sea Buckthorn (Hippophae rhamnoides L.) Seeds

作者：Marie Sajfrtova; Ivana Likova; Martina Wimmerova; Helena Sovova; Zdenk Wimmer

来源：International Journal of Molecular Sciences, 2010.

DOI：10.3390/ijms11041842

• Hippophae rhamnoides L.
• sea buckthorn seed
• fractionation in time
• supercritical carbon dioxide
• &beta
• -sitosterol

Supercritical fluid extraction represents an efficient and environmentally friendly technique for isolation of phytosterols from different plant sources. Sea buckthorn ( Hippophae rhamnoides L.) seeds were extracted with supercritical carbon dioxide at pressures ranging from 15每60 MPa and temperatures of 40-80 ～C. Oil and 汕-sitosterol yields were measured in the extraction course and compared with Soxhlet extraction with hexane. The average yield of 汕-sitosterol was 0.31 mg/g of seeds. The maximum concentration of 汕-sitosterol in the extract, 0.5% w/w, was achieved at 15 MPa, 40 ～C, and a carbon dioxide consumption of 50 g/g of seeds. The extraction rate was maximal at 60 MPa and 40 ～C. Both 汕-sitosterol yield and its concentration in the extract obtained with hexane were lower than with carbon dioxide.