Dahuang Zhechong Pill(大黄虫丸) Containing Serum Inhibited Platelet-Derived Growth Factor-Stimulated Vascular Smooth Muscle Cells Proliferation by Inducing G_1 Arrest Partly via Suppressing Protein Kinase C α-Extracellular Regulated Kinase 1/2 Signaling

作者：刘娜; 刘俊田; 姬媛媛; 路培培

来源：Chinese Journal of Integrative Medicine, 2012, 18(05): 371-377.

• Dahuang Zhechong Pill
• proliferation
• cell cycle
• cyclin D1
• p27

<正>Objective:To investigate effects of Dahuang Zhechong Pill(大黄(?)虫,DHZCP) on the cell cycle and the related signal pathways in vascular smooth muscle cells(VSMCs) stimulated by platelet-derived growth factor(PDGF) with the method of serum pharmacology.Methods:DNA synthesis in VSMCs was examined by detecting 5'-bromo-2'-deoxyuridine incorporation with the immunocytochemical method.The cycle of VSMCs was evaluated with flow cytometry.Expressions of cyclin D1,p27,protein kinase Cα(PKCα),and phosphorylated extracellular signal regulated kinase 1/2(ERK1/2) were quantified by Western blot method. Results:DHZCP containing serum significantly inhibited DNA synthesis of PDGF-stimulated VSMCs,arrested the cells in G_1 phase,modulated the protein expressions of cyclin D_1 and p27,and suppressed the activation of PKCαand ERK1/2.Conclusion:DHZCP containing serum inhibits VSMCs proliferation via modulating the expressions of cell cycle proteins to arrest the cell in G_1 phase,which is attributed to,at least in part,suppressing PKCα-ERK1/2 signaling in VSMCs.