CONA and UEA-I Ligands During Tooth Development Reveals Differences between Human and Rodents Models and Shows the Beginning of Mineral Extracelular Matrix Secretion Ligandos CONA y UEA-I Revelan Diferencias entre Humanos y en Modelos Roedores Durante el Desarrollo Dental y Muestran el Comienzo de la Secreci車n de la Matriz Mineral Extracelular

作者：Deborah Pitta Paraiso Iglesias; Moacyr Jesus Barreto de Melo Rego; Eduardo Isidoro Carneiro Beltro; Ana Paula Veras Sobral

来源：International Journal of Morphology, 2012.

• Odontogenesis
• Hidratos del carbono
• Histoquimica con lectinas
• Odontogenesis
• Carbohydrates
• Lectin histochemistry

Many of Glycobyological aspects of human development remain still unclear, mainly in oral science, and this could be observed in a lack of literature with few and old papers about this subject. During tooth histo-morphogenesis changes occur in basement membrane composition, expression of signaling molecules and in localization of cell surface components, where glyco components could be involved. In this sense, this work aimed to analyze the ConA ligands commonly founded in glycoproteins cores and UEA-I ligands since fucolization is a Key event in many physiological and pathological processes. Therefore 15 jaws of human fetuses were ethically obtained, histologicaly processed and then submitted to lectin histochemistry with appropriated controls. The results showed that lectins staining increase their intensity during dental development; ConA only starts to recognize glucose/mannose residues on ectomesechymal cells in the crow phase revealing its ligands when the enamel matrix starts to be secreted. Interestingly, Con A ligands were not founded in the basement membrane of the stratum intermedium of the enamel differing from rodents models. The staining pattern of UEA-I was different, starting to be positive in the ectomesenchyma since the bud stage and shown variable expression in cell type and staining intensity, which appeared be directly proportional to the progress of odontogenesis. Thus, this work shows that Con A and UEA-I exhibit a growing staining directly proporcional to ameloblasts and odontoblasts cytodiferenciation and revels some glycan differences between human odontogenesis and rodents models. Los aspectos glicobiol車gicos del desarrollo humano siguen siendo poco investigados, sobre todo en odontolog赤a, y esto puede ser observado en la literatura por los escasos y antiguos art赤culos sobre el tema. Durante la histomorfog谷nesis del diente se producen cambios en la composici車n de la membrana basal, en la expresi車n de mol谷culas de se alizaci車n y en la localizaci車n de la superficie celular de los componentes, donde los glico componentes podr赤an estar involucrados. Este trabajo tuvo como objetivo analizar los ligandos de la ConA ya que glucosa/manosa son com迆nmente encontrados en n迆cleos de glicoprote赤nas y ligandos de la UEA-I debido a fucolizaci車n es un evento clave en muchos procesos fisiol車gicos y patol車gicos. Fueron obtenidas 15 mand赤bulas de fetos humanos, procesadas y tratadas mediante histoqu赤mica de lectinas con controles apropiados. Los resultados mostraron que la intensidad de tinci車n de las lectinas aumenta durante el desarrollo del d