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摘要
In this study, ethanol extracts (ESs) and water extracts (WSs) were obtained from Spatholobus suberectus. The inhibition of α-amylase and α-glucosidase were determined. The results showed that the inhibition effect of ESs was significantly higher than that of WSs. To further explore the inhibition of the two enzymes by S. suberectus, the ESs was further separated by different polar solvents and obtained four components; W-ESs, D-ESs, E-ESs, and N-ESs. The different inhibitory effects of ESs and the four components at the same concentration range were as follows, α-amylase; W-ESs > D-ESs > ESs > N-ESs > E-ESs; α-glucosidase; W-ESs > ESs > N-ESs > E-ESs > D-ESs. W-ESs showed the best inhibition effect for both enzymes, with IC50values of ( 0.88 ± 0.02) mg/mL and (15.82 ± 2.79) (μg/mL. The Lineweaver-Burk curve was plotted to determine the type of enzyme inhibition. The results showed that WSs, ESs, W-ESs, and N-ESs were sensitive to α-amylase, which were the type of anti-competitive inhibition. D-ESs and E-ESs were competitive and non-competitive mixed inhibition. For α-glucosidases, WEs, D-ESs and E-ESs were the type of anti-competitive inhibition, while WSs, ESs and N-ESs were competitive and non-competitive mixed inhibition type. Our results showed that S. suberectus had the potential to be developed as a hypoglycemic drug. ? 2023 Science and Technology of Food Industry.
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