Backbone dynamics of TFE‐induced native‐like fold of region 4 of Escherichia coli RNA polymerase σ70 subunit

作者：Piotr Kaczka; Agnieszka Polkowska‐Nowakowska; Krystyna Bolewska; Igor Zhukov; Jarosaw Poznański; Kazimierz L Wierzchowski

来源：Proteins: Structure, Function, and Genetics , 2010, (3): NA-NA.

DOI：10.1002/prot.22607

• RNA polymerase
• sigma70
• NMR
• 15N relaxation
• trifluoroethanol
• HLHTH motif

Abstract(#br)Folding of a recombinant protein rECσ 70 4 comprising domain 4 of E. coli RNA polymerase σ . By all the criteria applied, three highly populated α‐helical regions separated by much more flexible residues forming a loop and a turn in the DNA‐binding HLHTH motif were identified. The locat...

• 单位
  Polish Academy of Sciences