Action of the medicine Canova on peritoneal resident macrophages infected with Trypanosoma cruzi = A o do medicamento Canova em macr車fagos peritoniais residentes infectados por Trypanosoma cruzi

作者：Vanessa Tagawa Cardoso de Oliveira; Daniela Maira Cardozo; Marcia Machado de Oliveira Dalalio; Silvana Marques Araujo

来源：Acta Scientiarum - Health Sciences, 2008.

• homeopathy
• Trypanosoma cruzi
• Canova
• homeopatia
• Trypanosoma cruzi
• Canova

Approximately 20 million of people are chronically infected withTrypanosoma cruzi in Latin America. The present work investigated the action of the homeopathic medicine Canova on in vitro experimental infections with T. cruzi Y strain, using Swiss mice resident peritoneal macrophages. Our results demonstrated that Canova induced a decrease in the production of H2O2 and TNF-a at 20 and 40% concentrations when compared to the control RPMI. However, when compared with this medicine excipient, a significant decrease in these mediators was observed with Canova at 40% concentration only. The production of NO and phagocytic activity were not affected. TNF-a inhibits T. cruzi replication in peritoneal macrophages in vitro, becoming an important agent of infection control by this parasite. Within this context, Canova , unlike what has been reported with other infections, would function as a stimulator of the infection, since it inhibited the production of TNF-汐 by peritoneal resident macrophages in vitro. Further studies should be carried out with elicited macrophages, in order to confirm the inhibitoryactivity of Canova on the production of TNF-汐 and other mediators in macrophages infected by T. cruzi. Aproximadamente 20 milh es de pessoas s o cronicamente infectadas pelo Trypanosoma cruzi na Am谷rica Latina. O presente trabalhoinvestigou a a o do medicamento homeop芍tico Canova em infec es experimentais ※in vitro§ com Trypanosoma cruzi, cepa Y, usando macr車fagos residentes peritoniais de camundongos Swiss. Os resultados indicaram que Canova induz a diminui o significativa da produ o de H2O2 e TNF-汐 em concentra es de 20 e 40%, quando comparado com ocontrole RPMI. Quando comparado com o excipiente do medicamento, observou-se diminui o na concentra o destes mediadores apenas na concentra o de 40%. A produ o de NO e a atividade fagoc赤tica n o foram afetadas. TNF-汐 inibe a replica o do protozo芍rio em macr車fagos peritoniais ※in vitro§, mostrando-se importante agente para o controle da infec o pelo parasita. Portanto, o medicamento Canova poderia estimular o processo de infec o, pois promoveu inibi o da produ o de TNF-汐 por macr車fagos peritoniais residentes ※in vitro§. Estudos adicionais devem ser realizados com macr車fagos elicitados, afim de confirmar a atividade inibit車ria da Canova sobre a produ o de TNF-汐 e outros mediadores em macr車fagos infectados por T. cruzi.