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摘要
Based on the target, the Shahe Reservoir, in the upstream of the north canal, the research analyzes the structure of the microorganism group in sediment and three typical pathogenic bacteria (E. coli, Enterococcus, Shigella) of gene expression level, the relation between environment factors using the ways of the modern analytical biology by collecting the samples in the surface(0~20 cm) of the Shahe Reservoir and columnar sediment.The study shows that the absolute gene expression level of the E. coli in sediment is relatively higher than the relative gene expression level in the downstream of the reservoir and contaminated area on horizontal distribution, the gene expression level of the Enterococcus is higher in the center area of the Shahe Reservoir, the gene expression level of the Shigella is the highest in the contaminated area.The absolute gene expression level of the three pathogenic bacteria in the sediment tends to decrease with the increasing of the depth on vertical distribution, but the relative gene expression level appears crest value in 10~30 cm. The relative gene expression level of the three pathogenic bacteria is much bigger in the sediment of the Shahe Reservoir with the structure of horizontal groups Clortridium sensu stricto, unclassified Anaeroineaceae, Povalibacter and the Anaeroineaceae is much bigger in the group structure of the vertical. The relative analysis of the Pearson suggests that both E.coli and TN(p<0.01), Shigella and TP(p<0.05) are positive correlation apparently on horizontal distribution; E. coli and TN(p<0.05), organic matter(p<0.05), Shigella and TP(p<0.05)are positive correlation on vertical distribution.The results clearly show that the amount of the pathogenic bacteria in the sediment in Shahe Reservoir is most likely to the water eutrophication.
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单位环境模拟与污染控制国家重点实验室; 中国科学院大学
