科研之友
微信
新浪微博
Facebook
分享链接
摘要
We report the production and characterization of endo-汕-1, 4-glucanase from isolated phytopathogenic bacterium Enterobacter cloacae. The bacterium was grown on different carbon sources including carboxymethyl cellulose (CMC) and 2% Avicel, for the production of endo-1, 4-汕 每D-glucanases enzyme. E. cloacae produced maximum levels of cellulases after 96 h of fermentation. Higher levels of endoglucanases were produced when microbe was grown on CMC. Endo-1, 4- 汕-D- glucanase had optimum pH and temperature of 5.8 and 40～C. The enzyme was inactivated by calcium chloride and a reducing agent 汕-mercaptoethanol.
