胸膜肺炎放线杆菌的实时荧光PCR检测

作者：李树清; 易建平; 李健; 胡永强; 王巧全; 陈志飞; 周筱华; 潘晓钟; 罗满林; 陈敏

来源：畜牧兽医学报, 2005, (09): 969-973.

• 胸膜肺炎放线杆菌
• 实时荧光PCR
• 检测 Actinobacillus pleuropneumoniae
• real-time PCR
• detection

Primers FP/ RP and TaqMan-MGB Probe were designed from sequence of apxIVA gene specific to all serotypes of Actinobacillus pleuropneumoniae. A real-time PCR method was developed for detecting Actinobacillus pleuropneumoniae, which can amplify all strains of APP. The sensitivity is 12fg DNA or 5.1CFU...

• 单位
  华南农业大学; 上海出入境检验检疫局