科研之友
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摘要
Objective:To evaluate the effect of Zhuanggu Jianxi Decoction(壮骨健膝方,ZGJXD)on interleukin-1β(IL-1β)-induced degeneration of chondrocytes(CDs)as well as the activation of caveolin-p38 mitogenactivated protein kinase(MAPK)signal pathway,investigating the possible molecular mechanism that ZGJXD treats osteoarthritis.Methods:Serum pharmacology was applied in the present study,where ZGJXD was orally administrated to New Zealand rabbits and then ZGJXD containing serum(ZGJXD-S)was collected for following in vitro experiments.CDs were isolated aseptically from New Zealand rabbits and then cultured in vitro.Upon IL-1βstimulation,the degeneration of CDs was verified by inverted microscope,toluidine blue stain and typeⅡcollagen immunocytochemistry.After IL-1β-stimulated CDs were intervened with blank control serum,ZGJXD-S,together with or without SB203580(a specific inhibitor of p38 MAPK)for 48 h,caveolin-1 protein expression and the phosphorylation level of p38 were determined by Western blotting,and the mRNA expression of IL-1β,tumor necrosis factorα(TNF-α),matrix metalloproteinase 3(MMP-3)and MMP-13 were examined by real-time polymerase chain reaction.Results:IL-1βstimulation induced degeneration of CDs,increased caveolin-1 expression and p38 phosphorylation,up-regulated the mRNA level of IL-1β,TNF-α,MMP-3 and MMP-13.However,the IL-1β-induced activation of caveolin-p38 signaling and alteration in the expression of p38 downstream target genes were suppressed by ZGJXD-S and/or SB203580 in CDs.Conclusion:ZGJXD can prevent CDs degeneration via inhibition of caveolin-p38 MAPK signal pathway,which might be one of the mechanisms that ZGJXD treats osteoarthritis.
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