科研之友
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摘要
Pyropia yezoensis is an important economic macroalgae cultivated in the coastal areas of northern China. In recent years, increased temperature and other environmental factors caused rotten diseases frequently, which restricted seriously the sustainable development of the aquaculture of P. yezoensis. We studied the effects of temperature (15 °C and 20 °C) and salinity (20, 25, and 30) on photosynthetic parameters, activity and transcription of antioxidant enzymes of P. yezoensis thallus. Results show that condition of 20 °C and salinity 20 resulted in the decrease of potential maximum quantum yield (Fv/Fm) and the increase of quantum yield of nonregulated energy dissipation Y(NO). Meanwhile, under the condition, the photosynthetic system of P. yezoensis was seriously affected as indicated by obviously increased content of malondialdehyde (MDA). On the other hand, with the decrease in the salinity, the activities of catalase (CAT) and ascorbate peroxidase (APX) decreased significantly, while the activities of superoxide dismutase (SOD) and peroxidase (POD) increased dramatically but increased slightly when salinity decreased, indicating that salinity effect was small. SOD transcription level was up-regulated slightly at 20 °C, while POD transcription level was down-regulated significantly with decrease of the salinity. In addition, under high temperature and low salinity stress condition (20 °C, salinity 20), the expression of key genes of antioxidant enzyme system was significantly down-regulated under high temperature and low salinity stress (20 °C, 20), and the scavenging of oxygen radical species was inhibited, which eventually led to the oxidative damage of cells. Therefore, under the conditions of high temperature and low salinity, the inhibition of antioxidase activity and related gene expression might explain the reason in biomolecular of the rotten diseases in aquaculture of P. yezoensis.
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