石菖蒲根茎化学成分及抗炎活性研究

作者：Qiu Lu-Ya; Yang Gang; Jin Qiong; Luo Xiao-Dong; Liu Ya-Ping^*; Wang Yi-Fen^*

来源：Chinese Traditional and Herbal Drugs, 2022, 53(15): 4617-4624.

DOI：10.7501/j.issn.0253-2670.2022.15.004

• 3,5-dimethoxy-4-hydroxy-propiophenone
• 4-(ethoxymethyl) phenol
• Acorus tatarinowii Schott
• anti-inflammatory activity
• inflammatory factors
• LPS induction
• trans-coniferyl aldehyde

Objective To study the chemical compositions and their anti-inflammatory activities from the dried rhizoma of Acorus tatarinowii. Methods The ethyl acetate layer of A. tatarinowii was phytochemically investigated by multiple step chromatography techniques, such as silica gel, YMC, Sephadex LH-20 gel and other column chromatography techniques. The structures of these isolates were unambiguously elucidated by means of extensive spectroscopic and comparison with corresponding data of the reported literature. All the isolated compounds were evaluated for their anti-inflammatory effects via detecting inflammatory mediator releases (IL-6、IL-1β、TNF-α) and NO medium level in RAW 264.7 macrophage cells induced by lipopolysaccharide (LPS). Results A total of 18 compounds, isolated from ethyl acetate layer of A. tatarinowii, were identified as protocatechualdehyde (1), 3,5-dimethoxy-4-hydroxypropiophenone (2), 4-(ethoxymethyl) phenol (3), pentyl (2E)-3-(4-hydroxy-3-methoxyphenyl)-2-propenoate (4), E-ferulic acid docosyl ester (5), E-ferulic acid eicosyl ester (6), 4-hydroxy-3-methoxy cinnamic acid ethyl ester (7), 3-(2-methoxyethyl)phenol (8), trans-coniferyl aldehyde (9), 4-methoxyphenol (10), β-asarone (11), α-asarone (12), 2-acetoxyacorenone (13), p-hydroxybenzoic acid (14), 5,8,11,14,17-eicosapentaenoic acid (15), (-)-syringaresinol (16), (-)-pinoresinol (17) and daucosterol (18). Conclusion Among all the isolates, compounds 2—9 are isolated from Acorus species for the first time. Furthermore, compounds 1—4, 6—12, 14—16 could remarkably inhibited the secretion of inflammatory mediators (IL-6、IL-1β、TNF-α) and NO medium level in LPS-stimulated RAW 264.7 macrophages cells at the concentration of 20 μg/mL, comparable to DXM used as the positive control. All the results suggested that A. tatarinowii possessed the anti-inflammatory activity.+ ? 2022 Editorial Office of Chinese Traditional and Herbal Drugs.

• 单位
  中国科学院昆明植物研究所; 云南中医药大学; 植物化学与西部植物资源持续利用国家重点实验室