科研之友
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摘要
Osteoarthritis is the more frequent cause of disability in adult people and it is associated to cartilage degeneration of affected joints. This cartilage has a limited ability to repair. Several treatments have been tested including the use of Mesenchymal Stem Cells. These cells are an attractive source for cartilage repair because of their availability to chodrogenic differentiation by progressing sequentially through the expression of cartilage specific extracelullar matrix molecules, as in the embryologic human development. The aim is to obtain, culture and differentiate rabbit Bone Marrow derived Mesenchymal Stem Cells in vitro to chondral lineage. By differential centrifugation the mononuclear cell level was obtained from rabbit bone marrow samples. This level was cultured until 70% confluence. Chondrogenic differentiation was performed in an aggregate culture system with TGF-b1. Sample quantity, culture efficiency, confluence time of cultures and differentiation quality were all evaluated. An average sample of 14.5 ml per side was obtained, culture efficiency was 80%, and average confluence time (70%) was 18 days. Differentiation culture had an 80% efficiency and optimal differentiation quality. Rabbit Bone Marrow derived Mesenchymal Stem Cells culture is a reproducible technique and by the use of an adequate methodology chondrogenic cells can be obtained in vitro. This model permits the study of chondral differentiation process and could have direct clinical application. This is the first successful Latin-American report in Mesenchymal Stem Cells culture and chondrogenic differentiation. La osteoartritis es la causa m芍s frecuente de discapacidad en personas adultas y se asocia a la degeneraci車n de los cart赤lagos de las articulaciones afectadas. Este cart赤lago tiene una capacidad limitada a la reparaci車n. Muchos tratamientos han sido probados, incluyendo el uso de c谷lulas madre mesenquim芍ticas. Estas c谷lulas son una fuente atractiva para la reparaci車n del cart赤lago debido a su disponibilidad a la diferenciaci車n condrog谷nica progresando de forma secuencial a trav谷s de la expresi車n de mol谷culas de la matriz del cart赤lago espec赤ficos extracelular, como en el desarrollo humano embrionario. El objetivo es obtener, el cultivo y la diferenciaci車n c谷lulas madre mesenquim芍ticas de conejo procedentes de m谷dula 車sea al linaje condral in vitro. Por centrifugaci車n diferencial a nivel de c谷lulas mononucleares se obtuvieron muestras de m谷dula 車sea de conejo. Este nivel se cultiv車 hasta su confluencia al 70%. La diferenciaci車n condr車genica se realiz車 en un sistem
