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摘要
Cellulase is a group of complex enzymes, which degrades cellulose into glucose and is widely used in biomass degradation, fuel production, and other fields. In this study, using microcrystalline cellulose as the sole carbon source, cellulase production of Trichoderma koningiopsis 8985 and that of T. reesei QM9414 were compared at different time points under liquid state fermentation. The cellulase produced by strain 8985 was detected 12 h after inoculation, and its filter paper cellulase activity reached 50% of the maximum 36 h after inoculation. However, the cellulase activity of QM9414 was not effectively induced at the time. The activities of filter paper cellulase, carboxymethyl cellulase, β-glucosidase and xylanase of the strains at 84 h of fermentation were tested, and those of strain 8985 reached 1.06, 3.62 and 1.80 and 6.67 IU/mL which were 1.72, 1.70, 6.35 and 1.12 times of those of strain QM9414, respectively. The optimal pH and temperature of filter paper cellulase activity of strain 8985 was 4.5 and 50 °C, and the activity was stable in the presence of Fe3+ (≤4 mmol/L) and Cu2+ (0-10 mmol/L).
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单位真菌学国家重点实验室; 中国科学院大学
