科研之友
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摘要
O objetivo deste estudo foi avaliar a utiliza o dos m谷todos de cultivo de escarro, Ogawa-Kudoh (O-K) e Petroff com tempo de descontamina o reduzido (Petroff- R), na rotina laboratorial de diagn車stico da tuberculose pulmonar. Um total de 459 amostras de escarro, de pacientes sintom芍ticos respirat車rios, foram cultivadas utilizando os m谷todos O-K e Petroff-R, paralelamente ao m谷todo cl芍ssico de Petroff. Os m谷todos O-K e Petroff-R mostraram-se eficientes na detec o de Mycobacterium tuberculosis e demicobact谷rias n o pertencentes ao complexo M. tuberculosis inclusive em amostras com baciloscopias negativas. N o houve diferen a estat赤stica entre a contamina o observada no m谷todo de O-K e Petroff. Estes dados sugerem que o m谷todo O-K, por ser de f芍cil execu o, baixo custo, apresentar baixo risco de contamina o do manipulador e boa sensibilidade na detec o de M. tuberculosis representa excelente recurso na rotina bacteriol車gica para o diagn車stico da tuberculose pulmonar. This study aimed to evaluate different methods for culturing sputum, Ogawa-Kudoh (O-K) and Petroff, using reduced time for decontamination(Petroff-R), expecting to know their effectiveness in tuberculosis laboratory diagnosis. Four hundred and fifty-nine sputum samples of patients with pulmonary symptoms were cultured using O-K and Petroff-R, to be compared with the regular Petroff method. O-K and Petroff-R methods showed efficient sensitivity in isolating Mycobacterium tuberculosis and micobacteria other than tuberculosis, even in samples with negative acid fast smear. Statistics analysis showed similar contamination rate for O-K when compared to Petroff.These data suggest that O-K, being easy to perform, having low costs, presenting low risk of worker contamination and good sensitivity in detecting M. tuberculosis, should be used in clinical laboratories as a good resource at tuberculosis diagnosis.
