辅酶Q10对鱼藤酮诱导的SHSY-5Y细胞凋亡效应的保护作用研究

作者：邓晟; 张伟; 尹桃; 唐翎; 邓昊

来源：中国临床药理学与治疗学, 2007, (10): 1177.

• Coenzyme Q10
• rotenone
• cell viability
• apoptosis

AIM: To determine whether the use of an antioxidant reagent coenzyme Q10 can protect human dopaminergic cell line SHSY-5Y against rotenone-induced apoptosis. METHODS: Cell viability was quantified by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and the apoptosis induced by rotenone was observed by Hoechst 33342, propidium iodide, and calcein AM staining in SHSY-5Y cells. RESULTS: Rotenone, a commonly used natural pesticide, inhibitor of mitochondrial respiratory chain complex I, was shown to decrease cell viability and induce apoptosis in SHSY-5Y cells. Pretreated with coenzyme Q10, the electron transporter in the mitochondrial respiratory chain, remarkably increased cell viability as well as significantly reduced the percentage of apoptotic SHSY5Y cells induced by rotenone. CONCLUSION: Coenzyme Q10 has a beneficial effect in protecting against rotenone-induced apoptosis in SHSY-5Y cells. The results support the hypothesis that coenzyme Q10 is a component of the antioxidant machine...

• 单位
  中南大学