Histological and Histochemical Studies on the Effects of Methotrexate on the Liver of Adult Male Albino Rat Estudios Histol車gico e Histoqu赤mico del Efecto del Metotrexato en el H赤gado de Rata Macho Albina Adulta

作者：Mohamed Akram Al Motabagani

来源：International Journal of Morphology, 2006.

• Metotrexato
• Hepatocitos
• Lobulos hepaticos
• Glicogeno
• Actividad enzimatica
• Methotrexate
• Hepatocytes
• Hepatic lobules
• lycogen
• Enzyme activity

Methotrexate (MTX) is widely used in the therapy of various types of malignancy. The present work was designed to investigate the histological and histochemical changes in the liver of albino rat following methotrexate administration. Fifteen adult male albino rats were used in the present work. They were divided into three main groups: Group I was kept without treatment and served as control. Groups II and III were given intraperitoneal injections of normal saline and MTX, respectively, at a dosage of (0.5 mg/Kg) twice weekly for total durations of 3, 6 and 9 weeks. The rats were sacrificed and the livers were excised and processed for histological and histochemical study. Examination of sections of the livers of group III showed mononuclear cell infiltration and an increase in the amount of collagen fibers in the portal tracts. There were focal areas of liver cell necrosis with distortion of the normal hepatic architecture. Moreover, there was a gradual and progressive decrease of glycogen content in the hepatocytes. Furthermore, succinic dehydrogenase and alkaline phosphatase activity were also decreased. In addition there was an increase in acid phosphatase activities in the degenerated areas and loss of activities in areas of massive cellular necrosis. It was concluded that repeated injections of MTX causes hepatic damage of a definite magnitude. This hepatotoxicity progressed with increasing cumulative doses of methotrexate. The present study provided further evidence to the cytotoxic potency of this antifolate El metotrexato es ampliamente usado en la terapia de varias enfermedades malignas. El presente trabajo fue dise ado para investigar los cambios histol車gicos e histoqu赤micos del h赤gado de rata albina, despu谷s de administrar dicho f芍rmaco. Se usaron 15 ratas albinas, machos, adultas, que fueron divididas en 3 grupos: El grupo I no tuvo tratamiento correspondiendo al control. A los grupos II y III se les administr車, por v赤a intraperitoneal, una soluci車n salina normal y metotrexato, respectivamente, con una dosificaci車n de 0,5 mg por Kg de peso, dos veces por semana, con una duraci車n total de 3, 6 y 9 semanas. Las ratas fueron sacrificadas y los h赤gados extra赤dos y procesados para los estudios histol車gico e histoqu赤mico. El examen de los h赤gados del grupo III mostr車 infiltraci車n celular mononuclear y un incremento en la cantidad de fibras col芍genas en la v赤a portal. Hubo 芍reas focales de necrosis de c谷lulas hep芍ticas con distorsi車n de la arquitectura hep芍tica normal. Adem芍s, hubo un gradual y progresivo decrecimiento del contenido de glic車geno