Teor e composi o qu赤mica do 車leo essencial de Melissa officinalis L. in vitro sob influ那ncia do meio de cultura = Content and chemical composition of the essential oil of Melissa officinalis L. in vitro under the influence of the culture medium

作者：erika Soares Reis; Jose Eduardo Brasil Pereira Pinto; Luciana Domiciano Silva Rosado; Ricardo Monteiro Correa

来源：Acta Scientiarum - Agronomy, 2009.

• Melissa officinalis
• planta medicinal
• oleo essencial
• cultura de tecidos
• Melissa officinalis
• medicinal plant
• essential oil
• tissue culture

Com o objetivo de avaliar o efeito do meio de cultura no teor e composi o qu赤mica do 車leo essencial de Melissa officinalis, foi realizado o presente trabalho, que avaliaram tr那s concentra es do meio de cultura MS. O experimento foi implantado em delineamentointeiramente casualizado, com sete repeti es, e cada repeti o foi representada por uma amostra de 20 g de plantulas frescas. A extra o do 車leo foi realizada em aparelho de Clevenger modificado com dura o de 1h e 30 min. e a an芍lise qu赤mica foi realizada por cromatografiagasosa. Observou-se que o teor e a composi o qu赤mica do 車leo essencial de M. officinalis foram influenciados pela concentra o de sais do meio MS. As plantulas que se desenvolveram nos meios MS e MS/4 apresentaram maior teor de 車leo essencial, e os componentes majorit芍rios foram o geranial (25,23 e 16,21%, respectivamente) e o neral (24,5 e 20,53%, respectivamente). J芍 o componente majorit芍rio presente no 車leo de plantulas de melissa cultivadas em meio MS/2 foio acetato de nerila (18,69%). The present work was carried out with the objective of evaluating the effect of the culture medium in the content and chemical composition of the essential oil of Melissa officinalis, evaluating three concentrations of the MS culture medium. The experiment was performed in a completely randomized design, with 7 repetitions, where each repetition was represented by 20 g of fresh biomass. Oil extraction was accomplished using a Clevenger modified device for 1 hour and 30 minutes, and the chemical analysis was accomplished by gas chromatography. It was observed that the content and chemicalcomposition of the essential oil of M. officinalis was influenced by the concentration of MS medium. The plants grown in the MS medium and MS/4 presented higher content of essential oil, and the major components of these plants were geraniol (25.23 and 16.21%, respectively) and nerol (24.5 and 20.53%, respectively). The major component present in the oil of melissa plants cultivated in MS/2 was neryl acetate (18.69%).