EFFECT OF DIFFERENT CARBON AND NITROGEN SOURCES ON 汐- AMYLASE PRODUCTION FROM BACILLUS AMYLOLIQUEFACIENS

作者：Neeraj Sharma et al

来源：International Journal of Pharmaceutical Sciences and Research, 2012.

• &alpha
• -amylase
• Bacillus amyloliquefaciens
• Carbon sources
• Nitrogen sources
• Submerged fermentation

Amylases are among the most important enzymes in present-day biotechnology. The effects of various carbon and nitrogen sources on 汐-amylase production by submerged fermentation (smF) from Bacillus amyloliquefaciens was investigated. The study revealed that the levels of the 汐- amylase production varied greatly with the type and concentration of carbon and nitrogen sources used. The highest 汐-amylase production was obtained in medium supplemented with lactose at 5g/l concentration. The other carbon sources like sucrose and mannitol also favored the 汐-amylase production. Maximum enzyme production was found with Ammonium chloride (NH4Cl) as the nitrogen source at 6g/l concentration. Yeast extract and peptone also seems to be suitable for the production of 汐-amylase from Bacillus amyloliquefaciens.