交替三线性分解算法与HPLC-DAD相结合用于硝基苯类化合物的二阶校正

作者：丁玉洁; 吴海龙; 方冬梅; 崔卉; 孙翔宇; 夏阿林; 胡乐乾; 俞汝勤

来源：高等学校化学学报, 2005, 26(07): 1255-1257.

DOI：10.3321/j.issn:0251-0790.2005.07.042

• 交替三线性分解算法
• HPLC-DAD
• 硝基苯类
• 二阶校正

High performance liquid chromatography(HPLC) is one of the analytical techniques used most frequently. Its chief limitation lies in the need to avoid the overlap of chromatographic peaks as much as possible. In this paper, the overlapped chromatograms and spectra system of 1-chloro-2,4-dinitrobenzene and 3,5-dinitrobenzoic acid in the presence of 2,4-dinitrobenzoic acid were resolved by using the alternating trilinear decomposition(ATLD) combined with HPLC-DAD. Moreover, both were determined simultaneously, whose recoveries were(102.1±2.2)% and(97.6±3.6)%, respectively. The satisfactory results show that this experiment can be easily performed without paying out time-consuming and complicated procedures and quantifying accurately. It revealed the ATLD algorithm was one of the approaches to solve second-order calibration problem in HPLC-DAD.

• 单位
  化学生物传感与计量学国家重点实验室; 化学化工学院; 湖南大学