Computational identification of putative lincRNAs in mouse embryonic stem cell

作者：Liu, Hui; Lyu, Jie; Liu, Hongbo; Gao, Yang; Guo, Jing; He, Hongjuan; Han, Zhengbin; Zhang, Yan; Wu, Qiong^*

来源：Scientific Reports, 2016, 6(1): 34892.

DOI：10.1038/srep34892

• LONG NONCODING RNAS
• GENE ACTIVATION
• EXPRESSION
• SEQ
• TRANSCRIPTOME
• PLURIPOTENCY
• LANDSCAPE
• RECONSTRUCTION
• CHROMATIN
• CROSSTALK

As the regulatory factors, lncRNAs play critical roles in embryonic stem cells. And lincRNAs are most widely studied lncRNAs, however, there might still might exist a large member of uncovered lncRNAs. In this study, we constructed the de novo assembly of transcriptome to detect 6,701 putative long intergenic non-coding transcripts (lincRNAs) expressed in mouse embryonic stem cells (ESCs), which might be incomplete with the lack coverage of 5' ends assessed by CAGE peaks. Comparing the TSS proximal regions between the known lincRNAs and their closet protein coding transcripts, our results revealed that the lincRNA TSS proximal regions are associated with the characteristic genomic and epigenetic features. Subsequently, 1,293 lincRNAs were corrected at their 5' ends using the putative lincRNA TSS regions predicted by the TSS proximal region prediction model based on genomic and epigenetic features. Finally, 43 putative lincRNAs were annotated by Gene Ontology terms. In conclusion, this work provides a novel catalog of mouse ESCs-expressed lincRNAs with the relatively complete transcript length, which might be useful for the investigation of transcriptional and post-transcriptional regulation of lincRNA in mouse ESCs and even mammalian development.

• 单位
  哈尔滨工业大学; 城市水资源与水环境国家重点实验室; 哈尔滨医科大学