科研之友
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摘要
Objective To study the chemical constituents in the stems and leaves of Hainanhuci (Damnacanthus hainanensis) and their anti-rheumatoid arthritis activities. Methods The chemical constituents in the stems and leaves of D. hainanensis were isolated and purified by silica gel, ODS, Sephadex LH-20 gel column chromatographies and preparative HPLC. The chemical structures of all isolates were identified using physicochemical properties, spectroscopic analyses, as well as the comparisons with the reported data in literature. In addition, all isolates were evaluated for their anti-rheumatoid arthritis activities using MTS method, via measuring their anti-proliferative effects on synoviocytes in vitro. Results A total of 18 compounds were isolated from the methanol extract of the stems of D. hainanensis, which were identified as naucleidinal (1), 1,2,3,4-tetrahydronorharman-1-one (2), 19-O-methyl-3,14-dihydro angustoline (3), latifoliamide B (4), latifoliamide D (5), bacilsubteramide A (6), vinmajine I (7), naucleofficine D (8), 1-carbomethoxy-β-carboline (9), naphthisoxazol A (10), 1,6-dihydroxy-2-methyl-9,10-anthraquinone (11), rubiadin-1-methyl ether (12), 1,3,6-trihydroxy-2-methoxymethyl-9,10-anthraquinone (13), 3,6-dihydroxy-2-hydroxy methyl-9,10-anthraquinone (14), 7-hydroxychromone (15), 5,7-dihydroxychromone (16), 6,4'-dihydroxy-3'-methoxyaurone (17), and farnisin (18). Compounds 11–14, 17 and 18 displayed the inhibitory effects on the proliferation of MH7A synovial fibroblast cells with the IC50 values in range of (8.93 ± 0.09) to (152.58 ± 0.32) μmol/L. Conclusion All compounds are isolated from the genus Damnacanthus for the first time, and compounds 11–14, 17 and 18 exhibited remarkable anti-rheumatoid arthritis activities.
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