科研之友
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摘要
The cyclamate, a sweetner substance derived from N-cyclo-hexyl-sulfamic acid, is largely utilized as a non-caloric artificial edulcorant in foods and beverages as well as in the pharmaceutical industry. The objective of this study was to evaluate karyometric and stereological alterations in the rat fetal pancreas resulting from the intraperitoneal administration of sodium cyclamate. The exocrine pancreas of ten fetuses of rats were evaluated, five treated and five controls chosen at random, in which five rats that received from the 10th to 14th days of pregnancy an intraperitoneal daily injection of sodium cyclamate at 60 mg/Kg of body weight during 5 days. At the 20th day of gestation, the animals were removed and weighed, as were their placentas; the length of the umbilical cords also were measured. After the laboratory processing, semi-seriated 6mm cuts stained with haematoxyline and eosine were performed. In seven karyometric parameters (major, minor, and medium diameters, volume, area, perimeter, and volume-area ratio), the increase was statistically significant in the treated group when compared with control group. Stereological parameters showed in the treated group a significant increase in the cellular volume and a significant reduction in the numerical cellular density. These results showed that the sodium cyclamate in pregnant rats led to retardation of fetal development and hypertrophy in the exocrine pancreas of the rat fetuses. El ciclamato, es una substancia derivada del 芍cido N-ciclo-hexil-sulf芍mico, bastante usada como edulcorante no cal車rico en los alimentos y bebidas, as赤 como en la industria farmac谷utica. El objetivo de este estudio fue evaluar las alteraciones cariom谷tricas y estereol車gicos en p芍ncreas fetal de rata tras la administraci車n intraperitoneal de ciclamato de sodio. El p芍ncreas exocrino de diez de los fetos de rata fueron evaluados, cinco tratados y cinco controles seleccionados al azar, en el que cinco ratas recibieron del d赤a 10 al d赤a 14 de pre ez una inyecci車n intraperitoneal diaria de ciclamato de sodio a 60 mg/Kg de peso corporal durante 5 d赤as. En el d赤a 20 de gestaci車n, los animales fueron retirados y pesados, al igual que sus placentas. Asimismo, se midi車 la longitud de los cordones umbilicales. Despu谷s del procesamiento de laboratorio, cortes semi-seriados de 6米m, se ti eron con hematoxilina-eosina. En siete par芍metros cariom谷tricos (di芍metros mayor, menor y medio, volumen, 芍rea, per赤metro y relaci車n 芍rea/volumen). El aumento fue estad赤sticamente significativo en el grupo tratado comparado con el grupo control.
