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摘要
In order to study the molecular mechanisms of green tea polyphenols (GTPs) in treatment or prevention of breast cancer, the cytotoxic effects of GTPs on five human cel lines (MCF-7, A549, Hela, PC3, and HepG2 cels) were determined and the antitumor mechanisms of GTPs in MCF-7 cels were analyzed. The results showed that GTPs exhibited a broad spectrum of inhibition against the detected cancer cel lines, particularly the MCF-7 cels. Studies on the mechanisms revealed that the main modes of cel death induced by GTPs were cel cycle arrest and mitochondrial- mediated apoptosis. Flow cytometric analysis showed that GTPs mediated cell cycle arrest at both G1/M and G2/M transitions. GTP dose dependently led to apoptosis of MCF-7 cels via the mitochondrial pathways, as evidenced by induction of chromatin condensation, reduction of mitochondrial membrane potential (ΔΨm), improvement in the generation of reactive oxygen species (ROS), induction of DNA fragmentation, and activations of caspase-3 and caspase-9 in the present paper.
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