Copper(Ⅱ) ions-immobilized virus-like hollow covalent organic frameworks for highly efficient capture and sensitive analysis of amyloid beta-peptide 1–42 by MALDI-MS

作者：Wende Ma; Chao Zhong; Juan Lin; Zhuling Chen; Guorong Li; Wei Tong; Yijing Wu; Lan Zhang; Zian Lin^*

来源：Chinese Chemical Letters, 2022, 33(12): 5174-5179.

Amyloid beta-peptide 1–42(Aβ 1–42) is one of the biomarkers of Alzheimer’s disease, and its selective capture and quantitative detection are important for diagnosis and treatment of Alzheimer’s disease.Herein, copper(Ⅱ) ions-immobilized virus-like hollow covalent organic frameworks(V-HCOFs@Cu2+) were synthesized by a facile approach. The as-prepared V-HCOFs@Cu2+showed unique morphology, ultrahigh specific surface(2552 m2/g), uniform mesoporous structure(3.2 nm), superior chemical stability and abundant binding sites. Based on these excellent properties, the V-HCOFs@Cu2+could be adopted as an ideal enrichment probe for highly efficient capture of Aβ 1–42, exhibiting high adsorption capacity(320 mg/g), and fast adsorption equilibration time(3 min). In addition, an attractive approach of the V-HCOFs@Cu2+-based matrix-assisted laser desorption/ionization mass spectrometry(MALDI-MS) was developed for the rapid screening and quantitative analysis of Aβ 1–42 in human serum by using C-peptide as an internal standard, which exhibited low limit of detection(LOD, 0.2 fmol/μL), and satisfactory recovery. This work provides an alternative solution for enrichment of biomarkers and also offers the potential applications of COFs in clinical analysis.

• 单位
  福州大学