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摘要
Objective:To test whether Shenfu Injection(参附注射液,SFI)might attenuate the impact of cerebral energy dysfunction after resuscitation in a pig model of cardiac arrest(CA).Methods:Thirty-four Wuzhishan miniature inbred pigs were randomly divided into three groups:the SFI group(n=12),the saline group(SA group,n=12),and the sham-operated group(sham group,n=10).Following successful return of spontaneous circulation(ROSC)from 8-min untreated ventricular fibrillation,animals received a continuous infusion of either SFI(0.2 mL/min)or saline for 6 h.Cerebral performance category score was evaluated at 24and 48 h after ROSC,followed by positron emission tomography and computed tomography scans of cerebral glucose uptake.Surviving pigs were euthanized 48 h after ROSC,and the brains were removed for detecting mitochondrial function.Results:Compared with the SA group,SFI treatment produced a better neurologic outcome48 h after ROSC(P<0.05).However,there was no significant difference of survival rate between the SA and SFI groups(83.3%vs.81.8%,P>0.05).After ROSC,the SA group showed a decrease in the maximum standardized uptake value of different regions in the brain tissue,where SFI treatment can ameliorate these decreases(P<0.01or P<0.05).Improved mitochondrial respiratory properties and higher mitochondrial membrane potential were also found following SFI treatment compared with the SA group at 48 h after ROSC(P<0.05 or P<0.01).Conclusion:SFI treatment after resuscitation has significant neuroprotective effects against disruption of cerebral energy metabolism from CA by improving glucose uptake and by normalizing mitochondrial function.
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