科研之友
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摘要
Whatman41Paper electrophoresis employing Barbital buffer pH (9.0) at 300 volts for 3 hours separated the Vipera russelli (Indian Russell＊s viper) venom in its basic components. This lead preliminary experiment helped in the selection of an appropriate HPLC (Shim Pack CLC-ODS (M)) column in a reverse operation/separation mode using 75% Acetonitrile as an eluent. Subsequent reverse phase preparative HPLC fractionated, the crude Russell＊s viper venom into 10peaks, whose protein contents were quantitatively determined by the FCR method. Peak which showed the highest protein concentration was further subjected to SDS-PAGE electrophoresis, molecular weight determination and procoagulant activity profile.
