一种新型6色荧光25个STR基因座复合扩增体系研究（英文）

作者：白雪^*; 姚伊人; 叶健; Song Wen; Ding Guangshu

来源：中国法医学杂志, 2020, 35(03): 258-264.

To develop and validate a novel 6-dye STR(short tandem repeat) 25-plex DNA typing system for forensic DNA profiling and databases. In this study, a novel STR 25-plex DNA typing system that includes 24 autosomal STRs(D1 S1656, D2 S1338, D2 S441, D3 S1358, D5 S818, D6 S1043, D7 S820, D8 S1179, D10 S1248, D12 S391, D13 S317, D16 S539, D18 S51, D19 S433, D21 S11, D22 S1045, CSF1 PO, FGA, Penta D, Penta E, TH01, TPOX, v WA, D11 S4463) and Amelogenin was developed. Validation studies demonstrated the sensitivity, accuracy, and reproducibility of our novel STR 25-plex DNA typing system. The sensitivity of the STR 25-plex DNA typing system was demonstrated by the ability to obtain complete profiles from as little as 0.125 ng of human DNA. Specificity testing was demonstrated by the lack of cross-reactivity to a variety of commonly encountered animal species and microbial pool. For stability testing, full profiles were obtained with humic acid concentration ≤60 ng/μL and hematin ≤600μM. For forensic evaluation, the selected 24 autosomal STRs followed the Hardy–Weinberg equilibrium. Since 24 autosomal STRs were independent from one another, PM(Probability matching) was 3.5434×10-28, TDP(Total Probability of Discrimination Power) was 0.999 999 999 999 999 999 9999999 69863, and CEP(Cumulative probability of exclusion) was 0.999 99999 375. The new STR 25-plex typing system is sensitive, reproducible, and stable, therefore it is highly applicable for use in national DNA database and can help to facilitate international data sharing.