Estudo da incid那ncia de amostras cl赤nicas do g那nero Candida isoladas de diversos s赤tios anat micos = Study of the incidence of clinical samples belonging to the genus Candida obtained from different anatomic sites

作者：Luciana Furlaneto Maia; Ana Flavia Leal Specian; Daniella Salvadego Wilnerzon Thrn; Marcelo Tempesta de Oliveira

来源：Acta Scientiarum - Health Sciences, 2007.

• Candida
• prevalencia
• candidiase
• Candida
• prevalence
• candidiasis

A candid赤ase, principal infec o f迆ngica do ser humano, 谷 provocada por leveduras do g那nero Candida, que fazem parte da microbiota end車gena e ex車gena do corpo humano. O objetivo deste trabalho foi avaliar a presen a das esp谷cies de Candida spp, em diversos s赤tiosanat micos. Um total de 90 amostras cl赤nicas foram isoladas em 芍gar Sabouraud contendo antibi車ticos e identificadas em meio cromog那nico CHROMagar Candida . Deste total, 58 amostras foram provenientes de secre o vaginal, 17 de raspado de unha, 8 de escarro e 7 deraspado de pele. Das amostras de secre o vaginal, 89% corresponderam a esp谷cie C. albicans, seguido de esp谷cies de Candida n o-albicans, sendo 5,1% de C. krusei e 1,7% de C. glabrata, C. tropicalis e Candida sp., individualmente. Para as amostras isoladas de raspado de pele n o foi observada preval那ncia de C. albicans (28%). As esp谷cies Candida n o-albicans corresponderam a 70% das amostras, sendo distribu赤das em C. glabrata (14%), C. krusei (28%) e demais esp谷cies de Candida (28%). A preval那ncia de esp谷cies Candida n o-albicans foi tamb谷m observada para as amostras isoladas de raspado de unha, sendo que 29% foram caracterizadas como C. glabrata, 23% C. krusei e Candida sp., individualmente e 11% de C. tropicalis. Amostras de C. albicans, isoladas neste s赤tio anat mico, representaram somente 11%. Em amostras obtidas de escarro foi observada somente duas esp谷cies, com preval那ncia de C. albicans (75%) seguida de C. tropicalis (25%). As amostras identificadas como C. albicans em meio CHROMagar Candida , foram confirmadas quanto ao crescimento a 42oC e pelo emprego da t谷cnica de seminested PCR. Candidiasis is the main human fungal infection caused by yeasts of the genus Candida which are part of endogenous microflora of thehuman body. The aim of this study was to analyze the incidence of Candida species obtained from different infection processes. A total of 90 clinical samples were isolated in Sabouraud agar supplemented with antibiotics, and identified by CHROMagar Candida . Among them, 58samples were isolated from vaginal secretion, 17 samples were from nail, 8 were from sputum and 7 were from skin. From the former, 89% were identified as C. albicans, followed by non albicans species, being 5.1% C. krusei, 1.7% C. glabrata, 1.7% C. tropicalis and 1.7% Candida sp. For samples isolated from nail it was not observed prevalence of C. albicans (28%); species nonalbicans corresponded to 70% of the samples, being 14% C. glabrata, 528% C. krusei and 28%Candida sp. The prevalence of non albicans species was also observed from samples isolated fro