科研之友
微信
新浪微博
Facebook
分享链接
摘要
Even though manganese is a bioelement essential for metabolism, excessive manganese levels in water can be detrimental to fish development and growth. Therefore, the aim of this study was to evaluate the effects of Mn2+ (0, 0.5,1, 2, and 4 mg<middle dot>L-1) exposure for 30 d on the growth performance, growth hormone/insulin-like growth factor (GH/IGF) axis, hypothalamic-pituitary-thyroid (HPT) axis, and monoaminergic neurotransmitters of Epinephelus moara female xEpinephelus lanceolatus male(Yunlong grouper). Compared with the control and low Mn(2+)concentration groups of (0.5 and 1 mg<middle dot>L-1), the high concentration of Mn2+ (4 mg<middle dot>L-1) significantly reduced body weight (BW), body length (BL), weight gain rate (WGR), and specific growth rate (SGR), increased the feed coefficient rate (FCR) and mortality of Yunlong groupers (P < 0.05). Further, the levels of GH and IGF, along with the expression of ghra and ghrb were significantly reduced after exposure to 2 and 4 mg<middle dot>L-1 Mn(2+)for 30 d, whereas the expression of sst5 was significantly up-regulated after exposure to 2 and 4 mg<middle dot>L-1 Mn(2+)for 20 and 30 days. Moreover, Mn(2+)exposure increased thyroid hormone (T3) and thyroid stimulating hormone (TSH) contents, accompanied by increased mRNA levels of dio1 and dio2, however, the T4 level was decreased. Finally, dopamine (DA) and serotonin (5-HT) levels significantly decreased after long-term exposure to higher concentrations of Mn2+, and the levels their metabolites changed as well, suggesting that the synthesis and metabolism of DA and 5-HT were affected. Accordingly, changes in the GH/IGF and HPT axes-related parameters may be the cause of growth inhibition in juvenile groupers under Mn2+ exposure, indicating that the relationship between endocrine disorder and growth inhibition should not be ignored.
-
单位上海海洋大学; 中国水产科学研究院
