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摘要
Objective To clarify he clinical significance, diagnostic and prognostic values of circular RNA circ_0141633 and circ_0008234 in peripheral blood of pancreatic cancer, and analyze heir mpact on the biological function of pancreatic cancer cells. Methods The peripheral blood samples of 97 pancreatic cancer patients and 71 healthy controls were collected, and the expression of circ_0141633 and circ_0008234 was analyzed by real-time quantitative polymerase chain reaction (qRT-PCR). The relationships between the expression of circ_ 0141633 and circ_0008234 and clinicopathological characteristics and prognosis of pancreatic cancer were analyzed by chi-square test, K-M survival curves and Cox proportional hazards regression model. The area under curve (AUC), sensitivity and specificity of circ_0141633 and circ_ 0008234 in the diagnosis of pancreatic cancer were analyze dby receiver operating characteristic (ROC) curve. The effects of circ_0141633 and circ_0008234 on the proliferation, migration, invasion and epithelial mesenchymal transformation (EMT) of Bxpc-3 cells were analyzed by methyl thiazolyl tetrazolium (MTT) method, cell scratch est, Transwell nvasion and Western blot. Results The expression of circ_0141633 and circ_0008234 in peripheral blood of pancreatic cancer patients was higher than hose of healthy controls (allP<0.05). High expression of circ_0141633 and circ_0008234 was associated with higher clinical stage, lymph node metastasis and venous invasion, and were ndependent risk factors for poor prognosis in pancreatic cancer (all P <0. 05). The overall survival rate of patients with high expression of circ_0141633 and circ_0008234 was significantly lower than hat of patients with low expre ssion of circ_0141633 and circ_0008234 (all P < 0. 05) . The AUG of circ_0141633, circ_0008234, the combination of circ_0141633 and circ_0008234, CA19-9 in the diagnosis of pancreatic cancer was 0. 70, 0.67, 0. 88 and 0. 82, with sensitivity of 64. 32%, 60. 79%, 78. 22% and 73. 97%, respectively. The specificity was 68. 54%, 65. 46%, 81.65% and 79.41%, respectively. The diagnostic efficiency of combination was superior o CA19-9, circj) 141633 and circ_0008234 alone (all P < 0. 05) . Interfering with circ_0141633 and circ_0008234 alone could inhibit the proliferation, migration, invasion and EMT of Bxpc-3 cells, and the above nhibitory effect was more obvious after interfering with both of circ_0141633 and circ_0008234 (all P <0. 05). Conclusions The high expression of circ_0141633 and circ_0008234 in peripheral blood could be used as potential diagnostic and prognostic biomarkers of pancreatic cancer, and could promote the progression of pancreatic cancer. ? 2022 ＪｏｕｒｎａｌｏｆＣｈｉｎｅｓｅＰｈｙｓｉｃｉａｎ.
