Regulation strategies and microbial response characteristics in the transformation process from anammox to CANON [由anammox转为CANON工艺的调控策略及微生物响应特性]

作者：Mao W.; Yang R.; Wang X.^*; Zhang Z.; Wu J.; Chen S.

来源：Chinese Journal of Environmental Engineering, 2021, 15(7): 2488-2501.

DOI：10.12030/j.cjee.202103122

• Completely autotrophic nitrogen removal over nitrite
• Free ammonia
• Microbial characteristics
• Nitrogen removal rate
• Pathway of $ {
• rm{H}}^ +_4} $-N removal
• rm{N}}{{

To explore the microbial response characteristics during the start-up and the high-load operation phase and determine the effective regulation strategy of the completely autotrophic nitrogen removal over nitrite (CANON) process, a stable anaerobic ammonium oxidation (anammox) system was conducted by adjusting the environmental factors (DO, pH and free ammonia), gradually reducing the concentration of nitrite and increasing the concentration of ammonia, then its transformation to CANON process was completed. The results showed that when the free ammonia was 10~20 mg·L?1, DO was 0.2~0.5 mg·L?1, and pH was 7.0~7.2, the growth of nitrite oxidation bacteria was inhibited, the ammonia oxidation rate and nitrogen removal rate could be gradually improved to 0.98 and 1.60 kg·(m3·d)?1, respectively, and start-up of CANON was successfully completed. In addition, the NH+4-N transformation ratio of anammox and nitrification pathway was finally stable at about 0.73. High concentration (>1 800 mg·L?1) of ammonia increased the abundance of anammox, but had an opposite effect on the ammonium oxidizing bacteria. The Candidatus Kuenenia and Candidatus Brocadia were the main genera of anammox bacteria under the early stage of the start-up and high-load condition, respectively. However, the SM1A02, as a possible genus of anammox bacteria, and Nitrosomonas, as a genus of AOB, were always the main genera during the start-up.

• 单位
  中国科学院大学