科研之友
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摘要
O tipo de reprodu o sexuada da planta cubiu 谷 ainda discutido entre os pesquisadores. O trabalho teve como objetivos: avaliar o controle da poliniza o na frutifica o de cubiu, verificar o efeito do est芍dio de desenvolvimento do bot o floral e do hor芍rio na receptividade do estigma e na germina o in vitro de gr os de p車len. Anteras de flores de plantas cultivadas em casa-de-vegeta o foram maceradas e os gr os de p車len inoculados em placas Petri para avalia o do efeito da concentra o de sacarose, do hor芍riode coleta e do est芍dio das flores na germina o in vitro. DNA de amostras de folhas jovens foram purificadas, amplificados por marcadores RAPD. Os resultados indicaram que o ambiente de cultivo interferiu na forma o de flores, infloresc那ncias e frutos. O emprego de diferentes concentra es de sacarose n o promoveu modifica es no n迆mero m谷dio de gr os de p車len germinados. Pela an芍lise dos estigmas, verificou-se que a receptividade independe dos hor芍rios de coleta e do est芍dio das flores. Pelas an芍lises de marcadores RAPD, verificou-se a ocorr那ncia de variabilidade gen谷tica intra-varietal. Assim, conclui-se que o cubiu 谷 uma planta al車gama, por谷m pelas taxas de germina o dos gr os de p車len e pela receptividade do estigma h芍 ind赤cios de ocorr那ncia de autofecunda o. The type of sexual reproduction of the species amazonic named cubiu is until discussed among researchers. Thus, the present work had the goal to evaluate the control of pollination in the fruiting of cubiu, to verify the influence of the developmental stage of the floral buds and time of pollination on the stigma receptivity, and in vitro germination of the pollen grain. Anthers from the flowers grown inthe greenhouse were grinded and the pollen grains were placed in Petri dishes to evaluate the influence of sucrose, time of harvest and stage of flower development on the germination. A DNA sample from young leaves was purified, amplified by the RAPD technique. The results pointed out that the environment of growth interfered in the flower formation, inflorescences and fruiting. The use of different concentrations of sucrose did not affect the number of germinated pollen grain. The stigma analyses showed that its receptivity was inde-pendent from the time of harvest and stage of flower development.The RAPD analysis showed occurrence of intra-variety genetic variability. Thus, the cubiu is alogamic plant, but based on the rate of pollen germination and stigma receptivity the plant may have occurrence of self pollination.
