A pilot study on anti-tumor effect of complanatoside A [沙苑子苷 A 通过抑制血管生成抗肿瘤作用研究]

作者：Yu M.; Rezeng C.; Yang K.; Jia H.; Zhang H.; Wang Y.; Zou Z.

来源：Drug Evaluation Research, 2020, 43(1): 21-26.

DOI：10.7501/j.issn.1674-6376.2020.01.004

• anti-angiogenesis
• chorioallantoic membrane of chicken embryo
• complanatoside A
• molecular docking
• reverse seeking target

Objective To study the potential anti-tumor effect of complanatoside A. Methods Target Searching of the anti-tumor protein correlated with complanatoside A based on reverse seeking target method, and further validated the target protein through the CDOCKER molecular docking approach. Finally, to validate neovascularization of complanatoside A (0.2, 0.4 and 0.8 mg/mL), the model of chick embryo chorioallantoic membrane was established. Results DS reverse seeking target found that the protooncogene of ABL1, SRC and 3 protein of FGFR1, FGFR2 and TGF-β, which were involved in tumor angiogenesis, were regulated by complanatoside A. Moreover, complanatoside A significantly inhibited small blood and medium vessels growth in Chick Embryo CAM model (P < 0.05 or 0.01). Conclusion The results suggested that CA might be an anti-tumor active ingredient of Astragalus complanatus. The anti-tumor mechanism of CA was involving in regulating the tumors gene expression and significantly suppressing angiogenesis in Chick Embryo CAM model.

• 单位
  首都师范大学; 中国医学科学院北京协和医院; 浙江大学; 北京协和医学院