Adsorption Effects of Lignin on Different Enzyme Proteins [不同木质素对酶蛋白的吸附影响及酶活变化研究]

作者：Mou H.^*; Liu Y.; Huang J.

来源：Transactions of China Pulp and Paper, 2020, 35(4): 23-27.

DOI：10.11981/j.issn.1000-6842.2020.04.23

• Adsorption interaction
• Cellulase
• Lignin
• Xylanase
• Β-glycosidase

Two kinds of lignin obtained from alkali and water soluble pretreatment of eucalyptus were used as raw materials, and through DNS and pNPG colorimetry, the effects of two kinds of lignin on the adsorption and desorption of different enzyme proteins (cellulase, xylanase, and β-glucosidase) and the inhibition of lignin adsorption on the activities of different enzyme proteins were studied. The results showed that the effect of temperature on the adsorption capacity of alkali lignin was greater than that of odium xylene sulfonate (SXS) lignin. The experiments of different enzyme proteins adsorbed on two kinds of lignin showed that the adsorbed enzyme proteins on SXS lignin were more easily to be desorbed. After lignin adsorption, the enzyme activity was significantly reduced, and the higher temperature could not only enhance the adsorption capacity of the lignins to enzyme proteins, but also inhibit the activity of enzyme.

• 单位
  华南理工大学; 制浆造纸工程国家重点实验室