科研之友
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摘要
The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus) and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%), followed by P. grandis and P. barbatus (77%). P. barbatus genotypes from Passo Fundo and Porto Alegre showed a genetic similarity which was close to 100%, while the genetic similarity for P. barbatus genotypes from other locations was higher than 96%. Although a low variability among genotypes of this species was found in this study, RAPD markers allowed a clear differentiation among the analyzed genotypes, showing a 53% mean genetic similarity, with a high correlation value (r = 0.99), which proves a high agreement between the genetic similarity and clustering data. O g那nero Plectranthus abrange plantas de diversas esp谷cies, referidas como boldo, que s o utilizadas na medicina popular pelas suas propriedades antidisp谷pticas, analg谷sicas e estimulantes da digest o. Gen車tipos relacionados podem diferir amplamente na quantidade de princ赤pios ativos naturais, resultando no uso de plantas n o-apropriadas 角 sa迆de da popula o. O objetivo do presente estudo foi avaliar a diversidade gen谷tica interespec赤fica de quatro esp谷cies do g那nero Plectranthus (P. grandis, P. barbatus, P. neochilus e P. amboinicus) e intraespec赤fica de P. barbatus, coletadas em diferentes localidades da regi o Sul do Brasil, por meio da t谷cnica de RAPD (Random Amplified Polymorphic DNA). Foi observada maior similaridade gen谷tica entre as esp谷cies P. neochilus e P. amboinicus (80%), seguido de P. grandis e P. barbatus (77%). Gen車tipos de P. barbatus provenientes de Passo Fundo e Porto Alegre apresentaram similaridade gen谷tica pr車ximo a 100%, enquanto nas demais regi es foi superior a 96%. Embora no presente trabalho tenha sido detectada baixa variabilidade entre gen車tipos desta esp谷cie, a t谷cnica de RAPD permitiu clara separa o entre as quatro esp谷cies analisadas, apresentando uma similaridade gen谷tica m谷dia de 53%, com um valor de correla o alto (r = 0,99), demonstrando e
