科研之友
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摘要
Diagnosis of Hirschsprung disease (HSCR) is quite entirely based on the histopathological analysis of suction rectal biopsies. Identification of ganglion cells can be difficult sometimes, especially in Hirschsprung's allied disease (HAD) and in newborns. In this study, we developed a protocol using Bcl-2, RET and calretinin (CR) immunostaining to diagnosis and differential diagnosis of HSCR and HAD. Sample from a total of 168 HSCR patients, 45 HAD patients and 20 age-matched normal samples from patients with colorectal trauma or undergoing anorectal colostomy were collected from 2011 to 2015 and full-thickness tissues were obtained for evaluation. Our results showed that only Bcl-2 has the positive immunoreactivity in the degenerative and immature ganglion cells, CR showed negative immunoreactivity in the degenerative ganglion cells of myenteric plexus in the intestinal. Besides, RET showed positive immunoreactivity in both immature and mature ganglion cells. Combined immunohistochemical detection of CR and RET facilitates can be used for diagnosis and differential diagnoses of HSCR and HAD. And the specific expression of Bc1-2 and CR play an essential role in diagnosis of HAD and newborn HSCR.
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