Profiles of metabolic gene expression in the white adipose tissue, liver and hypothalamus in leptin knockout(Lep~(△I14/△I14)) rats

作者：Leijian Guan; Kaixuan Xu; Shuyang Xu; Ningning Li; Xinru Wang; Yankai Xia; Di Wu

来源：Journal of Biomedical Research, 2017, 31(06): 528-540.

• Lep~(△I14/△I14)
• microarray analysis
• white adipose
• liver
• hypothalamus

Leptin deficiency is principally linked to metabolic disorders. Leptin knockout(Lep~(△I14/△I14) Sprague Dawley rats created by CRISPR/Cas9 is a new model to study metabolic disorders. We used a whole rat genome oligonucleotide microarray to obtain tissue-specific gene expression profiles of the white adipose tissue, liver and hypothalamus in Lep~(△I14/△I14))and wild-type(WT) rats. We found 1,651 differentially expressed(enriched) genes in white adipose tissue,916 in the liver, and 306 in the hypothalamus in the Lep~(△I14/△I14) rats compared to WT. Gene ontology category and KEGG pathway analysis of the relationships among differentially expressed genes showed that these genes were represented in a variety of functional categories, including fatty acid metabolism, molecular transducers and cellular processes. The reliability of the data obtained from microarray was verified by quantitative real-time PCR on 14 representative genes. These data will contribute to a greater understanding of different metabolic disorders, such as obesity and diabetes.

• 单位
  生殖医学国家重点实验室; 东南大学; 同济大学