科研之友
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摘要
In the present study, the pumpkin seed oil was extracted by ultrasonic-assisted solvent method, and the extraction process was optimized based on the relative content of linoleic acid (LA) (Y1) and crude oil yield (Y2) by Box-Behnken-Matlab analysis. The results indicated that the ultrasonic time had no significant effect on the Y1 of pumpkin seed oil (P>0.05), while the solid-liquid ratio with petroleum ether as the solvent, extraction temperature and extraction time had significant effect on the Y1 of pumpkin seed oil (P<0.05). The optimal extraction parameters of pumpkin seed were obtained by Box-Behnken-Matlab analysis: the ultrasonic time was 15 min, the solid-liquid ratio was 1:38. 65, the extraction temperature was 25. 75℃, and the extraction time was 2. 7 h. Under the optimal conditions, Y1 and Y2 could reach (48. 23±0.11)% and (24. 83±0.12)%, respectively. When the extraction time was 2. 5~3 h, the solid-liquid ratio was 1:(34~39), and the extraction temperature was 22~27℃, larger theoretical values of Y1 and Y2 could be obtained at the same time in this process parameter range. Under these conditions, the total phenolic content in crude oil was (968. 71±13. 96)mg GAE/kg. The results indicated that Box-Behnken-Matlab analysis method could not only obtain the optimal process parameters of pumpkin seed oil with high relative LA content, but also obtain the optimal process parameters range when considering the relative LA content and crude oil yield, which provided a reference for the industrial extraction of high-activity pumpkin seed oil.
