Effect of Jinmaitong (筋脉通) Serum on the Proliferation of Rat Schwann Cells Cultured in High Glucose Medium

作者：屈岭; 梁晓春; 张宏; 吴群励; 孙连庆; 顾蓓

来源：Chinese Journal of Integrative Medicine, 2008, 14(04): 293-297.

• Schwann cells
• high glucose
• primary culture
• Jinmaitong
• proliferation
• Chinese herbs' serum pharmacology

<正>Objective:To investigate the effect of Jinmaitong(筋脉通,JMT)serum on the proliferation of rat Schwann cells(SCs)primarily cultured in high glucose medium.Method:SOs were primarily cultured in Dulbecco's minmum essential medium(DMEM control),50 mmol/L glucose medium(50 mmol/L Glu),75 mmol/L glucose medium(75 mmol/L Glu),as well as 50 mmol/L glucose medium,with different concentrations of JMT serum(undiluted,1:2 diluted and 1:8 diluted)and Neurotropin(Ntp), respectively.The proliferation of SCs under different conditions was detected by MTT.Result:SCs grew exuberantly in DMEM within 24-72 h,but slowed down at 96 h.The proliferation of SCs was inhibited in 50 mmol/L Glu and 75 mmol/L Glu after cultures of 48,72 and 96 h,which showed that both were significantly different compared to the control group(P<0.01).The inhibition was more significant in 75 mmol/L Glu than in 50 mmol/L Glu(P<0.05).Spearman's rho analysis revealed that the proliferation of SCs had a negative correlation with the concentration of glucose...

• 单位
  中国医学科学院北京协和医院; 北京协和医学院