Chemical constituents from Diaphragma Juglandis Fructus and its antitumor activities [分心木化学成分及其体外抗肿瘤活性研究]

作者：Chen L.; Ge C.-L.; Wei H.-Y.; Ma X.-L.; Mai D.-N.; Shi L.-L.

来源：Chinese Traditional and Herbal Drugs, 2022, 53(12): 3595-3600.

DOI：10.7501/j.issn.0253-2670.2022.12.004

• (+)-isolariciresinol
• Blumenol C
• Diaphragma Juglandis Fructus
• Hexahydro-demethoxycurcumin-A
• Juglanin B

Objective: To study the chemical constituents and their antitumor activities from Diaphragma Juglandis Fructus. Methods: The 95% methanol extract was isolated and purified by various chromatographic methods, and its structure was identified by modern spectroscopy and physicochemical properties. The in vitro inhibitory activities of the compounds on human cervical cancer cell line Hela and human gastric cancer cell line HGC-27 were detected by MTT method. Results: Sixteen compounds were isolated and identified as isosclerone (1), engelhardione (2), 5-dehydroxy-octahydro-demethoxy curcumin-A (3), (E)-3,3'- dimethoxy-4,4'-dihydr-oxystilbene (4), blumenol A (5), (+)-erythro-7-O-ethylguaiacylglycerol (6), threo-3-(4-hydroxy-3,5- dimethoxyphenyl)-3-ethoxypropane-1,2-diol (7), (6R,9R)-blumenol B (8), hexahydro-demethoxycurcumin-A (9), blumenol C (10), ethyl 3,4-dihydroxybenzoate (11), (+)-isolariciresinol (12), juglanin B (13), heptanoid (14), 4,17-dimethoxy-2-oxatricyclo [13.2.2.13,7] eicosa-3,5,7(20),15,17,18-hexaene-10,16-diol (15) and alternariol 9-methyl ether (16). Conclusion: All compounds are isolated from Diaphragma Juglandis Fructus for the first time. Compounds 9 and 13 show inhibitory effect on Hela cells with IC50 of 25.33 and 16.29 μmol/L, respectively. Compound 13 shows certain activity on HGC-27 cells.

• 单位
  中国科学院大学