科研之友
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摘要
Peroxidase (POD) from Ceratophyllum demersum was isolated, purified and characterized, and the degradation of bisphenol A (BPA) by this POD was investigated. POD from C.demersum was purified by ammonium sulfate precipitation and AKTA protein purification system. The POD maintained relative high activity at a wide range of temperature and pH, and the highest relative activity was at 70°C, pH 7 with 5 mmol/L of H2O2. When the POD was 10U/mL, 99.67% degradation rate of 0.02mmol/L BPA was achieved within 15min, and BPA was completely degraded when the activity was 25U/mL and above. The optimal range of degradation conditions were at 30~60°C and pH 5~7 with 1~5mmol/L of H2O2. The kinetics of enzymatic degradation was analysed under the optimal conditions. The Km value was 0.09 mmol/L and the maximum reaction rate was 9.71mmol/(L·h). After degradation by C.demersum POD, the BPA contaminated water showed no significant estrogen effect on zebra fish. Our results indicated that POD from C.demersum was not only high stable but also efficient in the degradation of BPA, which may explain why C.demersum can be an ideal tool for BPA phytoremediation in aquatic environment.
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