Effect and mechanism of tanshinone IIA on TGF-β1 induced human skin fibroblast proliferation [丹参酮IIA对TGF-β1诱导的人皮肤成纤维细胞增殖的影响及作用机制]

作者：Xu X.-Q.; Han B.; Lai J.-H.; Lin Y.-J.; Shi J.^*

来源：Chinese Traditional and Herbal Drugs, 2020, 51(18): 4685-4690.

DOI：10.7501/j.issn.0253-2670.2020.18.011

• CCK-8 method
• COL I
• Human skin fibroblasts
• Hypertrophic scar
• P-Smad2
• P-Smad3
• Proliferation inhibition
• Tanshinone IIA
• TGF-β1/Smad pathway
• VEGFA
• Western blotting
• Α-SMA

Objective: To study the effect and mechanism of tanshinone IIA on human skin fibroblasts cell (HSF). Methods: CCK- 8 method was used to determine the effect of different concentrations of TSA on the proliferation of HSF induced by TGF-β1. The plate cloning ability of HSF treated with different concentrations of TSA (2.5, 5, 10 and 20 μmol/L) for 48 h were analyzed by plate clonogenesis assay. The protein expression of TGF-β1/Smad signaling pathway proteins and α-SMA, VEGFA and COL I were further measured by Western blotting. Results: CCK-8 and plate clonognesis assay results showed that TSA significantly inhibited the proliferation and colony forming efficiency of HSF (P < 0.01). Western blotting results revealed that each concentration group of TSA significantly inhibited the protein levels of p-Smad2 and p-Smad3, and down-regulated the ratio of p-Smad2/Smad2 (P < 0.01). The ratio of p-Smad3/Smad3 was significantly decreased in 5, 10 and 20 μmol/L TSA groups. Additionally, the expression levels of α-SMA, VEGFA and COL I in HSF decreased significantly with the increase of TSA concentration (P < 0.01). Conclusion: TSA exhibits the inhibitory effect on proliferation of HSF, and its mechanism may be related to TGF-β1/Smads signaling pathway.

• 单位
  中山大学