Variabilidade gen谷tica de amostras de Salmonella Typhi isoladas de surto e de casos espor芍dicos ocorridos em Bel谷m, Brasil

作者：Bastos; Flavia Correa; Lima; Karla Valeria Batista; Sa; Lena Lilian Canto de; Souza; Cintya de Oliveira; Lopes; Maria Luiza; Ramos; Francisco Luzio de Paula

来源：Jornal Brasileiro de Patologia e Medicina Laboratorial, 2008.

DOI：10.1590/S1676-24442008000400006

• salmonella typhi
• typhoid fever
• pfge
• outbreak
• sporadic cases.

background: salmonella typhi is the causative agent of typhoid fever, illness characterized by fever, migraine, myalgia, arthralgia, diarrhea or constipation, which may have complications and cause death. in brazil, the typhoid fever is endemic in the northern and northeastern regions, with outbreaks occurring in scorching months. objective: to analyse and compare the genetic variability of s. typhi strains isolated from outbreaks and sporadic cases of typhoid fever occurred in the city of bel谷m (pa) between december 2005 and march 2006. material and methods: twenty samples of s. typhi were analyzed: 10 of them were isolated from an outbreak occurred in guam芍 neighborhood in bel谷m, between december 2005 and march 2006, and the other 10 were isolated from sporadic cases in different neighborhoods of the same city in the same outbreak period. the genetic characterization was performed by macrorestriction analysis of genomic dna with xbai enzyme defined by pulsed-field gel electrophoresis (pfge). results: the xbal-pfge analysis of the studied samples revealed a genetic similarity of 83% to 100%. conclusion: this study demonstrated the clonal relation between the s. typhi samples from the outbreak and from the sporadic cases of typhoid fever occurred in the city of bel谷m between december 2005 and march 2006.