解淀粉芽孢杆菌 DMBA-K4 高产胞外多糖的发酵条件优化及其抗氧化活性研究

作者：Kuang Jiahua; Huang Yanyan; Hu Jinshuang; Yu Jiajia; Zhou Qinyu; Zhao Shan; Liu Dongmei^*

来源：Food and Fermentation Industries, 2020, 46(22): 28-35.

DOI：10.13995/j.cnki.11-1802/ts.024343

• antioxidation
• Bacillus amyloliquefaciens
• exopolysaccharide
• fermentation conditions
• response surface

An exopolysaccharides (EPS) producing strain was isolated from Yunnan Puer tea. This strain was defined as Bacillus amyloliquefaciens DMBA-K4 by 16S rDNA. Single factor trials and response surface tests were performed to optimize its EPS yield, and its in vitro antioxidant activity was also investigated. The optimal fermentation conditions were as follow: the culture medium consisted of 4 g / L sucrose, 5 g / L tryptone, 10 g / L yeast, 5 g / L NaCl, pH 7. 6, inoculator amount of 3. 10% and fermented for 18 h. Under these conditions, the maximal yields of EPS increased by 155. 7% and reached 171. 31mg / L. In vitro antioxidant results showed that EPS had strong antioxidant activity. When the concentration of EPS was 5 mg / mL, the scavenging rate of DPPH free radical and ·OH was 78. 60% and 75. 47%, respectively. The research provide theoretical support to EPS industrialization and widen the utilization prospect of novel bio-polysaccharide. ? 2020 Authors.

• 单位
  华南理工大学